Wednesday, February 26, 2020

The Music Industry Term Paper Example | Topics and Well Written Essays - 1750 words

The Music Industry - Term Paper Example Before the internet had arrived, the distribution of music was done through a few standards. These included CDs in the 1980s and the 1990s and before that, tapes formed one of the important mediums for the transmission of music to the final listener. All these standards had one feature in common. These mediums were traceable, secure and physical. Music theft and infringement was not common. Proper proceeds were given to the recording company, and henceforth to the artist. However, the sprawl of the internet led to websites, which started offering free music, which was available for download for everyone (Burgelman, Year). In this regard, the music industry started losing quite a lot in revenues. Some worrisome statistics will properly paint a picture of the loss of the music industry, because of an increase in digital media. Since peer-to peer, networking sites have evolved, around 47 percent of the music sales in the United States have dropped. Moreover, internet users consume aroun d $7 billion to $20 billion of the music, which is completely pirated, and the proceeds do not reach the right owner of the music. Around 30 billion songs illegally downloaded on the internet, through different websites in a span of five years (RIAA, n.d). ... Lastly, it tries to explain solutions to this problem in this dynamic and globalized world, where information is shared in nano-seconds. Concerning the legal issues involved in the music industry, it is important to discuss the matter of copyrights initially. Copyrighting is a way of providing legal protection, given to different forms of art, especially musical compositions such as lyrics, records and songs. In the music industry, the issue of copyrighting includes protecting the music itself (which includes lyrics and a melody) and the recording (such as mp3s, CDs, cassettes, DAT and other form of recording). If the music is recorded on a tangible medium, the creator has copyright protection. It is more prudent to register with the Copyright office, to gain maximum protection (Lawyers for the Creative Art, n.d). In this regard, distribution of music on the internet, without obtaining the permission from the original owner becomes a copyright infringement. The United States Copyrigh t Act states that the protection of a musical recording applies to sound recording. The reproduction of a particular sound recording forms the exclusive right of the Copyright owner. Infringement occurs when another party reproduces the musical recordings, without the permission of Copyright owner. Much of the music on the internet is obtained and transmitted without this permission. Therefore, it becomes illegal. However, not all the websites on the internet transmit the music illegally. These websites include iTunes, Rhapsody, MusicNet, and Liquid Digital Media among others. When an individual downloads music off this website, he or she has to pay a certain amount to the website (Bass, n.d). The mp3 technology or the

Sunday, February 9, 2020

Analysis of Ethnic Conflict in KOSOVO Essay Example | Topics and Well Written Essays - 2250 words

Analysis of Ethnic Conflict in KOSOVO - Essay Example Furthermore, the area needs proper management of these diverse societies so that such conflicts can be avoided since these altercations might escalate into a bloody civil war2. Such is the case of Kosovo, formerly a province of Serbia which have erupted into a bloody war towards the latter part of the 90’s. The carnage in the area was only halted by the intervention of NATO. Although this temporarily stopped the fighting and an independent state seems to be forming, the area remains in a precarious ceasefire as relative tensions remain between the Albanians and the Serbians wherein the secession resulted in the breakaway of an Albanian dominated Kosovo. This left the remaining Serbians in a quandary since they may be subjected to discrimination. The fear of possible persecution threatens to drive the Serbs away from Kosovo in effect dislocating them from their homes and towards a life of uncertainty3. Part 1 Kosovo Ethnic Conflict History has been teeming with ethnic conflicts and there have been numerous cases of violence that bordered on genocide. Through each of these conflicts, the diversity of culture as well as past excessiveness and offenses of one culture to another serve as fertile grounds for multi-ethnic conflicts to escalate into bloody wars. In Kosovo, the people of the neophyte state have been trying to lead a life of normalcy but as mentioned, considerable tension remains. After the breakaway, Kosovo was held by the majority Albanians that through the years have accumulated in the area. However, these people have experienced severe persecution when the autonomy of the then Serbian province has been revoked. There had been an apparent attempt to cleanse the area of Albanians when the ruling Serbs massacred numerous Albanians. This did not spare women, children and the elderly. Likewise, summary executions, kidnappings and arrests were rampant.4 The memories of these horrible events have been seared deep within the Albanians that now control Kosovo. As mentioned, there is a lingering anxiety within the remaining Serbs that have been relegated into the minority in the area. In a statement by Dr. Covic in front of the UN Security Council in New York over half a decade after NATO intervened and an uneasy ceasefire ensued, he advanced the concerns that the agreements have yet to be met by the Albanian authority. Dr. Covic lamented on the over 200,000 internally displaced Serbs and called on the council to act on these problems along with the need to implement court decisions in order to return properties to heir rightful Serbian owners. He likewise mentioned the seemingly disturbing solid line up of Albanians in the governing body of the area which may be detrimental to non-Albanians5. Hence, this essay will look into the situation of the now minority Serbs in Kosovo and the persisting multi-ethnic tensions between the two groups. Divisiveness and Scars of the Past The Albanians have constituted the majority of the populat ion in Kosovo long before the province split from Serbia in a bid to become independent. Previously, despite the lack of economic prosperity which was instrumental in driving most of the Serbs out of the area, the Albanians enjoyed a respectable degree of autonomy. This was shattered by the revocation of this autonomy paving the way for conflicts to arise. The human rights violations intensified and the massacres heightened to almost genocide

Thursday, January 30, 2020

A Case Against Slavery & Uncle Tom’s Cabin Essay Example for Free

A Case Against Slavery Uncle Tom’s Cabin Essay HB Stowe’s Uncle Tom’s Cabin was a powerfully evocative condemnation of slavery, and was famous in its own time. While many held that Stowe herself knew nothing about slavery or southern life, having never seen southern plantation life, it had its intended effect regardless: to stir up anti-slavery sentiment in the north, and to a lesser extent, in the South. Chapter VII is called â€Å"A Mother’s Struggle,† and it brings together many of the themes that dominate this book. First, the centrality of motherhood and the invincible power of a mother that is soon to be separated from her only son leads the action of this work. Second, the dominance of the Protestant Christian faith, a faith that serves to both assist slaves in tolerating their condition as well as providing them the strength to escape is the engine of the action. Since God is on the side of those who oppose slavery, ultimately, their struggle will emerge victorious and hence, the strength to persevere is present. Hence, this chapter is a central part of the work as it synthesizes the main themes of the book in rather stark terms, since this chapter is really the first â€Å"action† chapter of the book, that is, it is the beginnings of Eliza’s escape from her bondage and traces her moves northward to the Ohio river. The first real issue that drives Eliza to escape is the fact that she and her son are possibly to be sold. The Shelby family that owns them seeks to sell several slaves to pay off debts, and Eliza, who is not poorly treated by her mistress, Mrs. See more:  First Poem for You Essay Shelby, will not be separated from her only son (1-2). Here, the institution of slavery is shown in its worst aspect: the breaking up of families in the interest of the financial solvency of their masters. It was debated how often this was actually done, but this first few pages show the slave owning Shelby family as more or less humane, but forced due to circumstances to part with slaves, slaves, like Tom himself, who has befriended the son of the Shelby family. But stronger than all was maternal love, wrought into a paroxysm of frenzy by the near approach of a fearful danger. Her boy was old enough to have walked by her side, and, in an indifferent case, she would only have led him by the hand; but now the bare thought of putting him out of her arms made her shudder, and she strained him to her bosom with a convulsive grasp, as she went rapidly forward (1). This passage is an important part of the introduction to this chapter in that it places maternal power at the center of the war against slavery. It is this fear mixed with love that will keep Eliza moving forward. â€Å"Sublime is the dominion of the mind over the body, that, for a time, can make flesh and nerve impregnable, and string the sinews like steel, so that the weak become so mighty. † It is love, ths power of mind, the love for her son that keeps her moving forward, rather to face death, freezing, or torture if caught rather than be apart from her boy (2). It seems that, as Eliza continues her trek forward, that her body has become completely subsumed under her mind, and her love. Hence, she refuses to eat what she has packed for the trip North, and instead only wants her son to eat (3). But along with the power of a mother’s love, is the power of the belief in God and his Scriptures: â€Å"An hour before sunset, she entered the village of T , by the Ohio river, weary and foot-sore, but still strong in heart. Her first glance was at the river, which lay, like Jordan, between her and the Canaan of liberty on the other side. † (4). This passage is telling, in that it provides Old Testament references to the move toward liberty. The move of slaves northward to freedom is likened to the move of Israelites, themselves slaves to the Egyptians, over the Jordan river to the Canaanite valley which God has chosen for his people. For the slave treated harshly and not given even basic freedoms, the move northward was considered the promised land, the land of freedom, regardless of the hardships of slavery: this is the indictment of the institution as a whole. Slaves wold risk everything to avoid the institution, even a more or less comfortable existence that Eliza enjoyed with the Shelby’s. In this chapter, the mood is that if God closes one door, He opens another. So with Eliza gets to the river, the barrier between herself and freedom, it has been largely iced over (5). Given this, the ferry will not run. However, she is briefly taken in by a family connected with the ferry house on her side of the river. The action quickly and awkwardly shifts back to the slave quarters at Mrs. Shelby’s, where the slaves are speaking about the upcoming sale. Speaking of the slave trader, Chloe says, â€Å"He desarves it! † said Aunt Chloe, grimly; â€Å"hes broke a many, many, many hearts, I tell ye all! † she said, stopping, with a fork uplifted in her hands; â€Å"its like what Masr George reads in Ravelations, souls a callin under the altar! and a callin on the Lord for vengeance on sich! and by and by the Lord hell hear em so he will! † (7). This is very important, since it is clear that the slaves hold that God is on their side, not the other way around. On the other hand, this point of view is made more ambagious by Uncle Tom’s statement that damming people to Hell is not a very nice thing to do, and that eternal punishment is not a fit crime for selling slaves, which after all, is not eternal (7). This creates a concept of equivocalness where two factions of the slave population have two different points of view: the first, that of Chloe, that slavers are evil and will be punished to Hell, and the more moderate view of Uncle Tom, that slavers are victims of circumstance, who themselves, did not invent this institution. But Aunt Chloe responds, though indirectly to Uncle Tom: â€Å"Dont natur herself kinder cry out on em? † said Aunt Chloe. â€Å"Dont dey tear der suckin baby right off his mothers breast, and sell him, and der little children as is crying and holding on by her clothes, dont dey pull em off and sells em? Dont dey tear wife and husband apart? † said Aunt Chloe, beginning to cry, â€Å"when its jest takin the very life on em? and all the while does they feel one bit, dont dey drink and smoke, and take it oncommon easy? Lor, if the devil dont get them, whats he good for? † And Aunt Chloe covered her face with her checked apron, and began to sob in good earnest. (8) Again, Stowe is making another attack on slavery here, using the splitting up of families as her center line of argument. Since she is dealing with a family that treats their slaves moderately well, she cannot really rely on the physical violence argument so common in anti-slavery polemics. Since motherhood is at the center of this work, it seems that the basic argument against slavery is that since slaves have no freedom, they cannot fight back when their families are taken apart. Hence, Stowe holds that the family, especially the mother, is the institution of society that maintains moral norms and hence, will be the institution that takes slavery apart. If anything, this is the primary case against slavery being made here. Moving back to the action on the Ohio river, Eliza has been spotted by the slave trader, the very focus of evil throughout this book. It must also be pointed out that the trader and his style of life are equally repugnant to the Shelby’s (10). But as far as Liza is concerned, when she is spotted, A thousand lives seemed to be concentrated in that one moment to Eliza. Her room opened by a side door to the river. She caught her child, and sprang down the steps towards it. The trader caught a full glimpse of her just as she was disappearing down the bank; and throwing himself from his horse, and calling loudly on Sam and Andy, he was after her like a hound after a deer. In that dizzy moment her feet to her scarce seemed to touch the ground, and a moment brought her to the waters edge. Right on behind they came; and, nerved with strength such as God gives only to the desperate, with one wild cry and flying leap, she vaulted sheer over the turbid current by the shore, on to the raft of ice beyond. It was a desperate leap impossible (14). This passage is worth extended comment. First of all, as God has closed the door of the ferry, he has opened another, more dangerous door, that of skipping over the ice cakes. This seems powerfully symbolic: the ice represents the cold hearts of the slave traders and even the good slave owners. Under intense pressure, the power of family–of motherhood–takes over, what seems impossible is not so, if God is on your side. Slavery, it seems, will be defeated by slave mothers trampling on the cold hearts of the slave owners through material love and devotion to get to freedom. On the other side, Eliza meets a friendly farmer, Mr. Symmes, who assists in her escape. Stowe remarks on page 10, â€Å"So spoke this poor, heathenish Kentuckian, who had not been instructed in his constitutional relations, and consequently was betrayed into acting in a sort of Christianized manner, which, if he had been better situated and more enlightened, he would not have been left to do. † Again, a powerful statement is being made here: since slave owners were normally of the â€Å"gentlemanly† class, that is, the civilized class, civilization is equated with the dominating of slaves. Since poor whites were not slave owners (and treated only little better than the blacks) and were not part of their civilization, they had not â€Å"learned† about the inferiority of blacks and the racial and class hierarchy of the South. Therefore, their lack of civilization is a good thing, since simple common sense seems to dominate over â€Å"civilized manners. † Hence, the hero of the story (or at least of the chapter) is this simple farmer from Kentucky who does not represent civilization, but simple agrarian manners and common sense morality. Hence, in conclusion, Stowe is making several points here. First, that the real evil of slavery is the destruction of the family. If the family is the center of social life in that it is the bearer of tradition and moral values, then its destruction must lead to the beastialization of society. This might explain the evil of the slave traders, since they seem to specialize in breaking up families, and hence, are the very symbol of this beastialization. Second, a mother’s love, coupled with God’s force, will lead the southern slaves to freedom. Third, that freedom must be earned, and this is applicable to slaves and slave owners, both of which have not known freedom. Slave owners are as debased by slavery as the slave, since he is dependent upon them and hence, not free. Lastly, that nothing is impossible with God, and with a love of freedom. This is the case against slavery, in that it crushes all of these impulses, impulses that God as implanted in the human spirit and hence, are part of His creation, now enslaved by others. All quotes above are from the Virginia University edition of Uncle Toms Cabin, Published by the University of Virginia E-Text Center of the University of Virginia Library System. (http://etext. lib. virginia. edu/etcbin/toccer-new2? id=StoCabi. sgmimages=images/modengdata=/texts/english/modeng/parsedtag=publicpart=7division=div1)

Wednesday, January 22, 2020

Abortion: A Growing Concern Essays -- essays research papers

Abortion The issue of abortion causes discussions about human interactions where ethics, emotions and law come together. There are many reasons why women decide to have abortions. Whatever the reasons a woman decides to have an abortion, it seems only justified that she should be able to make decisions in regards to her life and body. The decisions that she makes will be beneficial to both the woman and the foetus because it will prevent many problems from occurring in the future. If a woman were to have an unwanted baby, she may neglect or even abuse the child causing the child to go into the care of the government. The government has no valid reason in bringing an unwanted life into the world. Bringing an unwanted child into the world is not helpful to the parents, the child and the government, because if the parents cannot take care of the child, eventually, the government will be responsible for the child. The right to have an abortion is personal and essential to a woman’s life, because having a baby in one’s life causes dramatic changes. Pregnancy and childbirth can prevent women from keeping and getting jobs. When women are pregnant many employers do not want to hire them because when the woman has a baby, the company or employer must still pay her for the time that she is out of work by law. Also, raising a baby and providing for all the needs that a child has is a lot of money, which can create an insufficient income for many women. Another factor that imposes on ...

Tuesday, January 14, 2020

Abortion Should Not Be Banned

Abortion is a widely debated topic that has been conflicting politicians and women around the world. Abaluck’s article, â€Å"Late-Term Abortion Should Not Be Banned† did not provide half as many sources or facts as Boland’s article, â€Å"Second Trimester Abortion Laws Globally. † Boland provided more statistics and alternant resources for the reader. It can look impressive if an author throws in an extensive reference list, but if he does not persuade you in an argument, all he has done is give you a history lesson. This is a perfect example of what Boland did in his essay. Of the two articles I researched, Abaluck’s article was more argumentative and forced me to question my own morals and view-points on abortion. Abaluck stated his opinion immediately by having his argument presented in his title. I loved this about his article because when I am reading something, it is hard for me to follow only numbers. I do not want a math lesson; I want my brain and beliefs to be challenged. I believe Abaluck’s article provides the necessary evidentiary support to persuade the audience to have mutual feelings about his stance on late-term abortion. There are two completely different vibes given off in each article. Abaluck’s article, â€Å"Late-Term Abortion Should Not Be Banned† was definitely my favorite because he gave an actual argument. With all of the facts he provided, he seems to know a significant amount of information about abortion and acts so passionate about it. However, Boland’s piece is the polar opposite of an argumentative article. This is one of the most informative pieces I have ever read and I feel much more aware of how other countries view abortion. I know this is the effect Boland was going for, and the article succeeded its purpose. Boland’s article was just straight facts and kind of boring. Although I was given more information in Boland’s article, I was not persuaded either way on the argument. The credibility of each author is high, but when it comes down to who I would want on my side in an argument, it would be Abaluck. Both authors work for Harvard in different areas. Jason Abaluck is the president of the Harvard Liberal Monthly Magazine and Reed Boland is a research associate at the Harvard School of Public Health. This spikes heir credibility through the roof and gives me no doubt that they know what they are talking about when it comes to research. Since Abaluck writes for a liberal magazine, I am sure his argument is a little biased considering his political views, but unlike Boland, he actually has an opinion. I am more apt to be pulled towards something with a little bit of passion and heart. Part of human nature is forming an opinion by listening to, reading, or watching someone else’s opinion and deciding if you agree or disagree depending on your moral beliefs. I believe late-term abortion should not be banned. A woman’s health should always be taken into consideration especially when there are expected complications. During a second trimester check up, you are likely to be able to see any abnormalities developing in your child. This is the time where it is not only crucial to be able to ensure your baby’s life is developing correctly, but also that the woman is remaining healthy. Most late-term abortions are not done just because a woman changes her mind about having a child. These abortions should not be banned because a lot of times, it is crucial to the woman’s life to have this done. Not only does this affect her physical well-being, it also affects her mentally. For the rest of any woman’s life, she will remember and think about the baby she â€Å"almost† had and she will likely require therapy. Yes, there is always the chance a woman is not having an abortion for all the right reasons, but the attachment that forms between a mother and child cannot be torn so easily. If late-term abortion were to be made illegal, a woman would have no choice but to have a child and risk her own life due to the known abnormalities of her child. The claim in Boland’s article is strictly informative. His article not only provides multiple, if not too many, statistics but also gives a table showing every country’s abortion laws. Maybe his intended audience was for people who were visiting the United States and wanted to see how their country lined up next to others. Most likely, he was writing this article just to inform whoever wanted to know about all the abortion laws worldwide. Regardless of who his audience is, he provides no information on what his personal view of abortion is. This fact made it hard for me to assess which article is better all around. I decided against Boland’s article because with all of the numbers and statistics, it was hard for me to follow along. If Boland had provided a little of his own opinion to spice things up, I would have gladly and openly considered his stance. The claim in Abaluck’s article was argumentative in that he gave his opinion and allowed no room for the other option. I liked that Abaluck’s article was argumentative rather than being strictly factual because he states his opinion, but was not pushy about it. Granted he does not acknowledge the other stand-point, he does not really make it seem like you should believe his argument. It is hard for an author to persuade their point without seemingly throwing it in your face, and I believe Abaluck demonstrated this perfectly. I was definitely persuaded by his article and took all of his ideas into consideration and even adjusted my view on the idea of late-term abortion. When beginning this assignment of researching my selected topic, I was completely against all types of abortion. Now, I realize there are a couple of reasons to reconsider this because you must always take the woman’s health into consideration. By including three personal stories in his article, I was forced to put myself in another person’s shoes and asked myself, â€Å"What would I do if this were me? † During the last few months of pregnancy, if the baby shows many signs of abnormalities or is going to threaten the life of the mother, late-term abortion should definitely be considered. If the unborn child stands little to no chance of survival, is it worth putting the woman’s life at risk? If Abaluck were to read Boland’s article, I believe it would only reinforce his viewpoint by giving him more reason to pull for not banning abortion. Since Boland does not provide an argument, it is easy to state that there is no disagreement shown when comparing the articles. If I were to sit down with both authors, I would first ask both of them to state their opinions and hopefully allow them to hash it out. It would not be difficult for me to assume that it would be an interesting debate knowing both writers know so much about the topic. Not knowing Boland’s stance makes it hard to assume that the two authors would argue, however I would love to assume there are at least a few differences in their beliefs. With Abaluck being a liberal, I am sure they would disagree on something. Or else, I hope they would because that would make sitting down with both of them way more entertaining. â€Å"When asked whether abortion should be legal, 80 percent of Americans say yes. When asked the same question about so-called ‘partial-birth’ abortion, 20 percent said yes. (Abaluck, 2007) Seeing this statistic, it is proven that terminology is crucial when presenting an argument. This proves that regardless of your argument, it would be easy to persuade an audience using harsh language like â€Å"partial-birth†. Even when I read an article against abortion, they used harsh terms like this and it made me resent people who would even consider having an abortion. However, once I learned the facts, I became aware that the language you use in an article is crucial to how you may persuade your audience. Abaluck brings this quote to your attention in the first paragraph of his article and clarifies the difference between real and harsh accusations against late-term abortion. By clarifying how to identify faulty terminology, Abaluck brings it to your attention that it is easy to sway people in your favor. I would undoubtedly say that Abaluck’s article was way better in not only getting a specific point across, but also persuading me and challenging my beliefs and morals. Being informed and having an opinion always go hand in hand and Abaluck’s article depicts this fact perfectly. On the other hand, Boland’s article was informative but very boring. To keep my attention, it is important to incorporate your opinion in order to challenge my beliefs. Although I was educated thoroughly on the topic of late-term abortion, I was not able to form an accurate argument after reading Boland’s article. He did not attempt to persuade me in either direction on the topic, so it was easy for me to determine the better of the articles.

Monday, January 6, 2020

Biology Of The Crispr Cas9 System - 866 Words

BIOLOGY OF THE CRISPR-CAS9 SYSTEM Functionality of the CRISPR system The endonuclease Cas9 is the core multifunctional protein of the CRISPR-Cas9 system. Two nuclease domains: HNH (39) and RuvC-like (40), within the Streptococcus pyogenes Cas9 (SpCas9) protein conduct the function of cleaving DNA strands. Studies have confirmed that the tracrRNA: crRNA duplex structure will lead to the sequence-specific DNA cleavage (41). PAM short sequence facilitates crRNA to recognize the targeted DNA sequence (42). Cas9 protein cuts the specific DNA strand that is complementary to the 20bp crRNA sequence via HNH domain; on the other hand, the SpCas9 RuvC-like domain cleaves the opposite targeted DNA strand (42) (Fig. 4). Mutating either of these two nucleases domains of SpCas9 will introduce a single-stranded DNA nick, while mutating both HNH and RuvC-like domains will transform SpCas9 into a RNA-guided DNA binding protein (42). X-ray crystallography and has confirm that endonuclease Cas9 undergoes a series of conformational rearrangements when it binds to the gu ide RNA and target DNA (55). With the development of the CRISPR-Cas9 technology, the duplex structure tracrRNA: crRNA has been engineered as one sgRNA retaining both tracrRNA and crRNA’s features. 20 nucleotides at the 5’ side of sgRNA will act as crRNA: recognizing and guiding SpCas9 protein to the targeted DNA sequences through Watson-Crick base pairing; whereas RNA forms a double-stranded structure at the 3’ end of sgRNAShow MoreRelatedDna Editing System For A Type II Crispr System Mechanism1295 Words   |  6 PagesCRISPR/Cas9 DNA editing system is a prokaryotic immune system that becomes resistant to foreign genetics such as plasmids. CRISPR stands for Clustered Regularly-Interspaced Short Palindromic Repeats, which are prokaryotic DNA strands that have a short repetition base sequence. Cas9 is a nuclease enzyme that is used to cut into DNA strands that are associated with CRISPR type II. CRISPR/Cas9 system has several advantages when using this technology in the biological and biomedi cal sciences. It alsoRead MoreAnalyzing The Genome Of Living Cells ( 1 )1139 Words   |  5 PagesBiology, in all of its glory, is quite amazing. It has always existed and always will; it merely just waits for a human to attempt to understand it. This understanding has taken centuries, however it seems to increase with the years. A very popular topic amongst biologists today is the genome, understanding it, mapping it, comparing one organism’s to another and so on. With the understanding of this genome though, we as humans want to delve into it, tweak it, and manipulate it until it is perfectionRead MoreGenes And Their Effects On Gene Editing Essay1428 Words   |  6 Pagesfields of molecular biology, as manipulated genes deem possible studying the specific genes and their effects. In the past, there were several attempts to manipulate gene function, including homologous recombination, RNA interference (RNAi), zinc-finger nucleases (ZFNs) and transcription-activator like ef fector nucleases (TALENs). These past approaches are expensive and time-consuming to engineer as opposed to Crispr Cas9, limiting their widespread use. What the Crispr Cas9 technique offers, andRead MoreThe Importance Of Stem Cell Research805 Words   |  4 Pagesnow. As of late, the technological innovation of CRISPR rises as a promising mechanism to revise hereditary variations from the norm. This method is being proclaimed for exactness and precision in hereditary altering. 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The story of this novel technique began in 1987 when Japanese researchers Nakata and his colleagues observed a series of 29nt DNA repeats interspacedRead MoreCRISPR-Cas9 Essay1058 Words   |  5 PagesMany researchers around the world have been using the CRISPR-Cas9 gene editing tool to try and fix or alter disease-causing mutations in human embryos. Many of these scientists have found a mutation in these genes known as, MYPBC3. This type of mutation mainly affects young athletes. It can cause the heart muscle to thicken, which is known as a heart condition called, hypertrophic cardiomyopathy. This type of condition is a dominant gene. Meaning, that if a child inherits this mutation, they willRead MoreBacterial CRISPR-Related Protein-9 Nuclease From Streptucoccus Pyogenes838 Words   |  4 Pagesbased on a bacterial CRISPR-related protein-9 nuclease (Cas9) from Streptococcus pyogenes is an effective and reliable method for making precise, targeted changes in the genome of living ce lls. The functions of the CRISPR (Short Palindromic Repeats Clustering Regular Intermediate Repeats) and CRISPR-associated (Cas) genes in adaptive immunity in selected bacteria and archetypes allow the organisms to respond to and remove invasive genetic material. Methods Three types of CRISPR mechanisms, the mostRead MoreEssay On SMA904 Words   |  4 Pages Impact Summary If this research is successful, it’s findings will increase awareness of research in SMA and introduces how CRISPR-Cas9 can be used as a simpler alternative to edit genomes. Currently we have only investigated the effect on SMN protein levels via the correction of the exon 7 sequence in the SMN1 gene. Therefore, the next stage will be to use CRISPR-Cas9 mediated gene editing to target different mutations associated with SMA. Ultimately, we aim to provide a form of treatment to alleviateRead MoreDelivery Of The Cas Components1306 Words   |  6 PagesDelivery of CRISPR-Cas Components: CRISPR systems are generally delivered in the cell using normal methods of transfection such as microinjection, gene gun, electroporation, sonoporation, and using viruses such as adenoviruses and lentiviruses. In cultured mammalian cells, researchers have used electroporation, nucleofection, and Lipofectamine mediated transfection methods to deliver vectors expressing the gene for gRNAs and Cas9 endonuclease. In cultured human and mouse cells, Lentiviral vectorsRead MoreThe And Viruses And Their Biological Function Essay1527 Words   |  7 PagesCRISPR loci are first identified in archaea and bacteria when they systematically drew attention from scientists with their biological function to fight phages and viruses (Hsu, Lander, Zhang, 2014). Structurally, a clustered set of Cas (CRISPR-associated) genes and a unique CRISPR array constitute the CRISPR loci. The CRISPR array was further comprised of short repetitive sequence interspaced by distinctive sequences (spacers) in correspond ence with exogenous genetic bits (protospacer). The natural

Saturday, December 28, 2019

Addiction is a disease Free Essay Example, 1750 words

Addiction has none of the above characteristics. (Dodes, 2011). The fact that, if addiction is not dealt with in time might get worse is the only characteristic of addiction that is similar to that of a disease. According to Mclemore (2007), one of the factors that lead to addiction includes genetic causes. Some types of addictions may be inherited. In case one comes from families with a history of addiction for example a child whose parents are alcoholics is most likely to be an addict although its not necessary that he/she becomes an alcohol addict he/she may become a workaholic or even a smoking addict. The environment can also be considered as a risk factor. For instance where one is raised increases or decreases the chances of one becoming an addict. In places where the substance is readily available and accessible, there is a possibility that there will be a high number of addicts. But in areas where the substances is not available or disapproved of by the society, the likelih ood that the number of addicts will be low is very high. We will write a custom essay sample on Addiction is a disease or any topic specifically for you Only $17.96 $11.86/pageorder now Young people between the ages of 14-18 that are addicts, in most cases come from homes where some of these addictions are considered as acceptable. Hence, in most cases they may not consider using some substances as wrong or even harmful. Addiction can also be mental, it may be caused by production of a chemical messenger in the brain know as dopamine. It is produced when someone thinks of the substance or activities that are associated with addiction. This chemical messenger activates a part of the brain that responds to rewards and the pleasure that makes the person have a good feeling. Repetition of introduction to these activities and substance makes one to be an addict. Addiction can also be caused by the need to perform better in school or even at work places. Some people have the belief that drugs enhance their performance in some activities that they undertake. Also the use of drugs can also be social based, people how are not able to social ize easily can turn to drugs so as to gain the courage to socialize. The use of a substance due to these beliefs, with time the person becomes an addict. According to Flowers (2010), some people also use addiction as a defense or coping mechanism due to past experiences which may be physical, psychological, emotional or sexual abuse. These encounters may cause someone to be prone to addiction so as to avoid dealing with some of these issues. They tend to rely on other substances or actions that might help them cope or avoid facing such experiences.